Data analysis with Wintrack - the basics
The purpose of Wintrack is the numerical and graphical off-line
analysis of paths and related data that were recorded from behaving
animals and then stored as case
documents. The process of data analysis involves three steps:
- The Arena Properties command is
used to define the arena
in which the experiment was carried out.
- A set of variables capturing the relevant aspect(s) of the data
is defined.
- The Analyze Case Document command
applies the variable definitions to the data and produces a numerical
or graphical result.
Wintrack offers two levels of data analysis. Quick an easy data
analysis has been implemented for those users who, using a simple
and intuitive interface, wish to carry out a rapid analysis of experiments
carried out in a standard open field, Morris watermaze or Barnes maze.
Advanced data analysis is aimed at those researchers who work
with nonstandard behavioral setups or are asking more specific questions
about their data.
The quick and easy way
- You first invoke the Arena properties
command. Its dialog box allows
you to select and scale one of three predefined arena types: open field,
water maze, or Barnes maze.
- Wintrack offers a long list of predefined
variables, so you do not need to worry about variable definitions.
- You open the case document(s) of interest and use the Analyze
Case Document command to analyze them, selecting and fine tuning
predefined variables from the list in
the Analyze Case Document dialog. The
results are displayed in a scrollsheet
that can easily be transferred to statistics and graphics software.
The advanced way
- The Wintrack power user begins by writing a custom
setup file (ASCII text file with extension .WSP) to define a
custom arena that may contain an almost unlimited number of fields
representing objects and sub/zones. He can also change the graphical
appearance of the path,
specify the graphical appearance of behavioral events,
and define timelines that represent the flow of time rather than
the animal's position in space. Furthermore, custom setups permit to
handle data from experiments that are conducted without a confined
arena (eg. homing pigeon releases). Once built, a custom setup file
is parsed by the Arena properties command.
- In a custom variable definition file
(ASCII text file with extension .VDF), the Wintrack power user next
defines the variables that refer to the custom arena and any object
or zone it contains. While building
a custom variable definition file, up to 4096 custom variables
can be defined. The flow of data inside
Wintrack can be directed in many different ways. It begins with the
definition of a data matrix, from which
four types of numerical variables can be derived: profile,
trial, case,
and surface variables. Data can also
be evaluated graphically using bullets
or by converting surface variables into surface
documents with the New Surface Document
command. Analysis is not restricted to the path of the animal, but
may include behavioral events (eg. grooming or sniffing) and
other supplemental data (eg. vertical activity or flight altitude).
Using conditional trial discarding,
the analysis may be restricted to data that meet a set of criteria.
For more efficient maintenance, variables may be defined as variable
blocks.
- Custom variables are applied to case documents using the Analyze
Case Document command. The results are displayed in a scrollsheet
that can be transferred to statistics and graphics software. The results
of bullet analysis are displayed directly on top of the case data, whereas
surface variables are reported in scrollsheets from which surface
documents can be created.
Becoming a Wintrack power user requires that you familiarize yourself
with the basic concepts behind path data analysis and that you
learn the statement syntax of custom setup and variable definition
files. But this will greatly expand the power and flexibility of Wintrack.
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